Preparation of test bacteria sheet for moisture barrier microbial penetration tester
The moisture barrier microbial penetration tester is suitable for evaluating the performance of materials in resisting the penetration of bacteria in liquids when subjected to mechanical friction. It is mainly used for medical surgical drapes, surgical gowns and clean clothes. Applicable standards: YY/T 0506.6, ENISO 22610 Main parameters: 1. Turntable speed: 60rpm±1rpm; 2. Test pressure: 3N±0.02N; 3. Outward wheel speed: 5～6rpm; 4. Timer setting range: 0～99.99min; 5. Total weight of inner and outer rings: 800g±1g. Plate preparation: Staphylococcus aureus ATCC29213 on tryptic soy agar at 36°C±Incubate at 1°C for 18-24h, inoculate 2-3 colonies into 3ml of tryptic soy broth, and inoculate at 36°C±Incubate at 1°C for 18-24h. Dilute 1:10 with peptone water to a concentration of 1×104CFU/mL~4×104CFU/mL, count the final bacterial suspension. Open the sterile bag and take out the polyurethane film still attached to the IQ, and place the wettable polyurethane film of the bacteria-carrying material sheet face up on the cleanliness plate. For ease of operation, cover the four corners of the bacteria-carrying material sheet with double-sided tape fixed on the plate. Use the petri dish cover as a template to form a corresponding area on the biofilm, apply 1.0 ml of Staphylococcus aureus suspension to this area, and then dry the bacterial sheet at 56°C for about 30min, using sterile glass during the drying period. The applicator continues to coat the bacterial suspension on the bacterial carrier film to make the bacterial liquid evenly distributed. Test principle: Put the test piece on the agar petri dish, place a piece of bacteria of the same size on the test piece, and then cover it with a piece of high-density polyethylene film with a thickness of about 10 microns, and use a tapered steel ring to seal the three-layer material ( The test material is at the bottom, the bacterial sheet is in the center, and the high-density polyethylene film is on the top), and the ring kit is placed on the agar petri dish on the turntable. The test finger acts on the material in a way that can move on the entire surface of the petri dish. , subjecting the material to the combined action of pressure and friction to simulate the possible stress and microbial penetration of the barrier material in the actual application process. The microorganisms on the bacterial sheet penetrate the test material and migrate to the surface of the agar medium. The penetration performance of the test material can be quantitatively evaluated by culturing the agar plate and counting the colonies.
GESTER International Co.,Limited have now make a decision to enlarge our company in other countries.
See reviews of the latest trend in textile testing equipment industry at GESTER Instruments, and see the best that work in just minutes! Visit us right away!
GESTER International Co.,Limited, a manufacturer of textile testing equipment, might emphasize less hassle or less wasted time rather than emphasizing reliability or quality.
It is never too late to have a new mindset and to get things moving in the right direction. Choose GESTER International Co.,Limited to be your quality provider.
Through our distribution and marketing competencies, GESTER International Co.,Limited provides creative, customized, solutions for our customers. As a result, we achieve superior profit growth as the textile testing equipment company of choice.
The air permeability tester is a high-precision testing instrument designed to evaluate the breathability of PPE materials, textile fabrics, non-woven fabrics, woven fabrics, and industrial filter media. It plays a critical role in quality control, product development, and laboratory research across multiple industries including PPE, textiles, automotive interiors, and filtration systems.
Modern instruments such as the GT-C27 Air Permeability Tester are developed with advanced sensor systems, automated control logic, and multi-standard compatibility to ensure reliable and repeatable results. Complying with global standards such as ISO 9237, ASTM D737, DIN 53887, and GB/T 5453, it guarantees internationally recognized testing performance.
With high automation, accurate pressure sensing, and flexible unit conversion systems, air permeability testing equipment significantly improves testing efficiency while reducing human error. It is widely used in PPE manufacturing, textile engineering, non-woven product development, and industrial filtration quality inspection.
Personal protective equipment must meet specific performance requirements before it can be used in demanding workplace environments. ISO, EN, and ASTM standards provide important test methods and performance requirements for different PPE categories. However, the applicable standard depends on the product, intended application, target market, and specific protection requirements.
This guide explains the role of ISO, EN, and ASTM standards in PPE testing and introduces common requirements for safety footwear, protective gloves, protective clothing, masks, and safety helmets. It also explains how laboratories can select suitable PPE testing equipment according to the applicable test method.
Choosing the right abrasion testing equipment is essential for obtaining accurate and repeatable wear resistance results. DIN Abrasion Tester and Taber Abrasion Tester are two widely used abrasion testing machines, but they are designed for different material types and testing purposes.
This article provides a detailed comparison between DIN Abrasion Tester and Taber Abraser, covering their working principles, specimen requirements, industry applications, standard compliance, technical characteristics, operation features and practical advantages. DIN Abrasion Tester is mainly designed for rubber, elastic materials, tires, conveyor belts and footwear soles, while Taber Abrasion Tester is suitable for flat materials such as coatings, textiles, plastics, leather, metals and decorative surfaces.
By understanding the differences between these two abrasion testing methods, laboratories can select the most suitable abrasion tester based on sample geometry, testing standards and production quality control requirements.
Quick links
Products